Seth Green

The Nerd / Batman / Robin / Aquaman / The Penguin / Martian Manhunter / Toyman / Scarecrow / Gorilla Grodd / Little Cheese / Swamp Thing / Abin Sur / Ganthet / Green Arrow / Hawkman / Jimmy Olsen / Various (voice)

Breckin Meyer

Superman / Mirror Master (voice)

Nathan Fillion

Green Lantern / Mr. Freeze (voice)

Alex Borstein

Wonder Woman / Giganta / Woman (voice)

Matthew Senreich

The Flash / Chillblaine / Brainiac / Wildcat (voice)

Alfred Molina

Lex Luthor / Firestorm / Mr. Banjo (voice)

Paul Reubens

The Riddler (voice)

Neil Patrick Harris

Two-Face / Black Manta (voice)

Kevin Shinick

Narrator / Captain Cold (voice)