Lew Ayres

Dr. James Kildare

Lionel Barrymore

Dr. Leonard Gillespie

Laraine Day

Nurse Mary Lamont

Shepperd Strudwick

Dr. Gregory 'Greg' Lane

Samuel S. Hinds

Dr. Stephen Kildare

Emma Dunn

Mrs. Martha Kildare

Nat Pendleton

Joe Wayman, Ambulance Driver

Walter Kingsford

Dr. S.J. 'Walter' Carew, Hospital Administrator

Alma Kruger

Molly Byrd, Superintendant of Nurses