Josh Keaton

Peter Parker/Spider-Man (voice)

Lacey Chabert

Gwen Stacy (voice)

James Arnold Taylor

Harry Osborn (voice)

Alan Rachins

Norman Osborn (voice)

Deborah Strang

Aunt May Parker (voice)

Ben Diskin

Eddie Brock (voice)

Dee Bradley Baker

Curt Connors/Lizard (voice)

Robert Englund

Adrian Toomes/Vulture (voice)

Crispin Freeman

Max Dillon/Electro (voice)