Ben Diskin

Nigel "Numbuh One" Uno / Hoagie Pennywhistle "Numbuh Two" Gilligan Jr. / D.C.F.D.T.L. (Voice)

Lauren Tom

Kuki "Numbuh Three" Sanban (Voice)

Dee Bradley Baker

Wallaby "Numbuh Four" Beatles / D.C.F.D.T.L. / Chip / Toiletnator / Mr. Fibb (Voice)

Cree Summer

Abigail "Numbuh Five" Lincoln / D.C.F.D.T.L. (Voice)

Maurice LaMarche

Father (Voice)

Ric Stoneback

Adult Hoagie

Sonnie Brown

Adult Kuki

Jeff Bennett

Mr. Boss (Voice)

Jennifer Hale

Computer / Mrs. Uno / Numbuh 86 / Numbuh 363 (Voice)